abstract：:Two recombinant mouse mammary tumor virus (MMTV) subunit vaccines have been constructed, in which linear sequences of the envelope gp 52 glycoprotein (EP3) or the superantigen (SAg) have been fused to single or multiple repeats of a T-cell epitope (P30) from tetanus toxin. Histidine tags or glutathione-S-transferase (...

journal_title：Molecular immunology

authors： Astori M,Kraehenbuhl JP

更新日期：1996-09-01 00:00:00

abstract：:In the adult mouse, the earliest thymocytes are derived from bone marrow-resident T lymphocyte precursor (pre-T) cells that immigrate to the thymus. There they undergo maturation through a series of developmental steps that include rearrangement and expression of the TCR genes, positive and negative selection, and fun...

journal_title：Molecular immunology

authors： Wang TG,Lybarger L,Soloff R,Dempsey D,Chervenak R

更新日期：1996-08-01 00:00:00

abstract：:The high affinity receptor for IgE, which mediates allergic reactions, is found exclusively on mast cells, basophils and epidermal Langerhans cells. It is composed of an alpha, beta and two gamma-polypeptide chains, inserted as a complex into the plasma membrane. The alpha and beta-subunits are produced only by these ...

journal_title：Molecular immunology

authors： Witthuhn BA,Faust C

更新日期：1996-07-01 00:00:00

abstract：:We have sequenced the TCRs from Ld-specific alloreactive T cell hybridomas, whose reactivities we have found to be quite representative of those of a primary dm2 anti-BALB/cJ mixed lymphocyte reaction. We find V beta 6, V beta 7, V beta 8 and V beta 10 gene segments. V alpha usage is diverse, although closely related ...

journal_title：Molecular immunology

authors： Louie KA,Ochoa-Garay J,Chen PJ,McKinney D,Groshen S,McMillan M

更新日期：1996-06-01 00:00:00

abstract：:A highly purified preparation of a monoclonal antibody to vasoactive intestinal peptide (VIP) was analysed by gel filtration. Three peaks of VIP hydrolysing activity were observed, corresponding to the 150 kDa tetramer IgG, 80 kDa dimer of the heavy and light chains (H-L dimer) and 25 kDa L chain monomer. The hydrolyt...

journal_title：Molecular immunology

authors： Li L,Sun M,Gao QS,Paul S

更新日期：1996-05-01 00:00:00

abstract：:The purpose of the present work was to determine the ability of BgTX peptides, corresponding to the various loops and exposed regions of alpha-bungarotoxin (BgTX) and representing regions that are recognized by B and/or T cells, to stimulate protective immunity in mice against in vivo challenge with BgTX. The BgTX LD5...

journal_title：Molecular immunology

authors： Dolimbek BZ,Atassi MZ

更新日期：1996-05-01 00:00:00

abstract：:The binding of CD40 ligand on activated T cells to CD40 on resting B cells induces the expression of costimulatory molecules B7-1 (CD80) and B7-2 (CD86). The induction of B7 molecules by CD40 ligand-CD40 interaction represents a critical step in rendering B cells competent for antigen presentation. The CBA/N mouse has...

journal_title：Molecular immunology

authors： Goldstein MD,Debenedette MA,Hollenbaugh D,Watts TH

更新日期：1996-04-01 00:00:00

abstract：:A panel of human T cell clones specific for measles virus was characterized and among them fusion protein-specific, DR1-and DP-restricted T cell clones were selected to study the processing and presentation of determinants borne by a viral membrane protein. Using two independent methods to assess the activation of T c...

journal_title：Molecular immunology

authors： Demotz S,Péléraux A

更新日期：1996-03-01 00:00:00

abstract：:The complete cDNA sequences of two bovine IgGs were obtained by RT-PCR cloning. The first-strand cDNA was prepared from an animal homozygous (A2/A2) for IgG2a; the resulting sequences of the two bovine IgGs reported here were identified as IgG2a(A2) and IgG1. These sequences, and their deduced amino acid sequences, ar...

journal_title：Molecular immunology

authors： Kacskovics I,Butler JE

更新日期：1996-02-01 00:00:00

abstract：:Ligation of the B cell Ag receptor (BCR) activates a protein-tyrosine kinase (PTK) and CD45 protein-tyrosine phosphatase (PTPase)-dependent signaling cascade that results in the activation of Ras. This pathway of Ras activation can operate independently of protein kinase C (PKC) activity. Activation of Ras may lead to...

journal_title：Molecular immunology

authors： Kawauchi K,Lazarus AH,Sanghera JS,Man GL,Pelech SL,Delovitch TL

更新日期：1996-02-01 00:00:00

abstract：:Secreted glycoproteins generally contain oligosaccharides of the complex type. However, several molecules have been described in which individual glycans are processed differently from one another. Folding, assembly and oligomerization could affect the maturation of certain glycans by hindering them to the Golgi proce...

journal_title：Molecular immunology

authors： Cals MM,Guenzi S,Carelli S,Simmen T,Sparvoli A,Sitia R

更新日期：1996-01-01 00:00:00

abstract：:A monoclonal human IgG1, Campath-1H, was digested with glycosidases to assess the effect of carbohydrate on the functional activities of an IgG1. Removal of the complete carbohydrate moiety abolished complement lysis activity and antibody-dependent cell-mediated cytotoxicity, but left antigen binding activity and prot...

journal_title：Molecular immunology

authors： Boyd PN,Lines AC,Patel AK

更新日期：1995-12-01 00:00:00

abstract：:We report the construction, expression and purification of a bispecific single-chain Fv antibody fragment produced in Escherichia coli. The protein possesses a dual specificity: the single-chain FvB1 portion is directed to the Idiotype of BCL1 lymphoma cells, the single-chain Fv2C11 moiety binds to the CD3 marker on T...

journal_title：Molecular immunology

authors： De Jonge J,Brissinck J,Heirman C,Demanet C,Leo O,Moser M,Thielemans K

更新日期：1995-12-01 00:00:00

abstract：:The isolation and characterization of two human serum proteins, RHP and N-RHP, are described. N-RHP appears to be the normal counterpart of RHP which is found at elevated levels in sera of patients with rheumatoid arthritis [Rosano et al. (1988b) Inflammation 12, 351 - 360]. Although both proteins crossreact with anti...

journal_title：Molecular immunology

authors： Hurwitz C,Rosano CL,Hechemy KE,Weber P,Parhami N

更新日期：1995-11-01 00:00:00

abstract：:The major histocompatibility (MHC) class I antigens are coordinately expressed in most cells. However, some tumors or virus-infected cells lack expression of one MHC class I antigen, while expression of the other MHC class I antigens is unaffected. We previously described the selective expression of MHC class I antige...

journal_title：Molecular immunology

authors： Luo H,Sopchak L,Lerman SP,King SR

更新日期：1995-10-01 00:00:00

abstract：:Peripheral blood B lymphocytes have been isolated from healthy individuals who were immunized with lymphocytes from HLA-incompatible donors and transformed with Epstein-Barr virus to produce human monoclonal cell lines specific for human HLA molecules. The cell lines have been previously characterized and are known to...

journal_title：Molecular immunology

authors： Andris JS,Abraham SR,Pascual V,Pistillo MP,Mantero S,Ferrara GB,Capra JD

更新日期：1995-10-01 00:00:00

abstract：:Apoptosis can be induced in the human leukemic T-cell line Jurkat when these cells are stimulated by several known T-cell activators. Among the stimuli that activate Jurkat cells, calcium ionophore A23187, phytohemagglutinin and cross-linked monoclonal antibody anti-CD3 are the best inducers of apoptosis. The activato...

journal_title：Molecular immunology

authors： Ruiz-Ruiz MC,Oliver FJ,Izquierdo M,López-Rivas A

更新日期：1995-09-01 00:00:00

abstract：:We have examined the regulation of the AP-1 DNA transcription complex during T cell activation in response to interleukin 1 (IL-1) and phorbol ester (TPA) treatment of the IL-1 responsive murine thymoma T cell line, EL4 6.1 C 10. IL-1 synergistically enhances the stimulatory effect of TPA on AP-1-mediated gene express...

journal_title：Molecular immunology

authors： Brooks JW,Yoza BK,Mizel SB

更新日期：1995-08-01 00:00:00

abstract：:A number of proteins with significant similarity to the tumour necrosis factor (TNF) have been identified over the last years. Upon interaction with their cognate receptor (members of the TNF-receptor family), all members of this protein family induce either cell death or proliferation/differentiation of the receptor-...

journal_title：Molecular immunology

authors： Peitsch MC,Tschopp J

更新日期：1995-07-01 00:00:00

abstract：:We describe several proteins that are components of the rabbit B cell receptor complex. Two proteins (37 kDa and 42 kDa) were found in non-covalent association with IgM expressed on B cells from peripheral blood. These proteins were also immunoprecipitated by anti-B29 (Ig-beta) and anti-mb1 (Ig-alpha) monoclonal antib...

journal_title：Molecular immunology

authors： Fitts MG,Metzger DW,Hendershot LM,Mage RG

更新日期：1995-07-01 00:00:00

abstract：:The cDNA coding for a hybridoma anti Shigella dysenteriae type 1 antibody (3707 E9) has been cloned, and sequenced. Binding patterns with fragments of the bacterial polysaccharide antigen had already been studied in detail. The VH sequence utilizes the VH441 gene, first identified amongst beta-(1,6)galactan-binding an...

journal_title：Molecular immunology

authors： Miller CE,Huppi K,Siwarski D,Karpas A,Newman A,Mainhart C,Glaudemans CP

更新日期：1995-06-01 00:00:00

abstract：:Major histocompatibility complex (MHC) class II molecules are cell surface glycoproteins and are known to display processed antigens on the surface of antigen presenting cells (APC). Within the APC, the loading of processed antigenic peptides to MHC class II molecules is known to take place in the endosomal compartmen...

journal_title：Molecular immunology

authors： Mukku PV,Passmore D,Phan D,Nag B

更新日期：1995-06-01 00:00:00

abstract：:The site of the murine IgG1 molecule that regulates catabolism has recently been shown to encompass amino acids that are located at the CH2-CH3 domain interface. The CH2 and CH3 domains are connected to each other by a relatively flexible "mini-hinge" region, and flexibility in this region could clearly affect the ori...

journal_title：Molecular immunology

authors： Kim JK,Tsen MF,Ghetie V,Ward ES

更新日期：1995-05-01 00:00:00

abstract：:In addition to the antigen receptor, resting T cells express a number of receptors that can be stimulated to generate proliferative signals. These "accessory" receptors require co-expression of the T cell receptor (TCR), suggesting that they channel their signals via secondary activation of the signal transduction fun...

journal_title：Molecular immunology

authors： Zhang XM,Berland R,Rosoff PM

更新日期：1995-04-01 00:00:00

abstract：:A monoclonal antibody (vpg15) has been described which can block infection with feline immunodeficiency virus (FIV) and which recognizes the feline homologue of CD9. In order to study the role of feline CD9 in infection with FIV we have molecularly cloned a cDNA encoding feline CD9 by R.A.C.E (rapid amplification of c...

journal_title：Molecular immunology

authors： Willett BJ,Neil JC

更新日期：1995-04-01 00:00:00

abstract：:Recombinant DNA techniques now allow the production of "mini-antibodies" called Fv fragments. These have been produced either as the independent variable domains of the heavy and light chains non-covalently associated in one-to-one stoichiometry or as single-chain gene products with the two domains linked by an interv...

journal_title：Molecular immunology

authors： Webber KO,Reiter Y,Brinkmann U,Kreitman R,Pastan I

更新日期：1995-03-01 00:00:00

abstract：:The lymphocyte antigen CD6 has been extensively studied in humans, but has not been reported in mouse. Here we describe the isolation and characterization of a cDNA clone encoding the murine homologue of the human lymphocyte antigen CD6. Comparison of the predicted amino acid sequence of human and murine CD6 shows tha...

journal_title：Molecular immunology

authors： Whitney G,Bowen M,Neubauer M,Aruffo A

更新日期：1995-01-01 00:00:00

abstract：:It is well established that aggregation of cell surface immunoglobulin is involved in signal transduction by cells of the immune system. It is less well understood what special properties of these cell surface aggregates are important in initiating the signal cascade. Several authors have proposed that cells respond t...

journal_title：Molecular immunology

authors： Posner RG,Dembo M

更新日期：1994-12-01 00:00:00

abstract：:Affinity-purified major histocompatibility complex (MHC) class II molecules are known to bind antigenic peptides in vitro. The percentage of MHC class II molecules occupied with such peptides is usually very low and varies significantly depending upon the sequence and size of a given antigenic peptide. The present stu...

journal_title：Molecular immunology

authors： Nag B,Mukku PV,Arimilli S,Phan D,Deshpande SV,Winkelhake JL

更新日期：1994-10-01 00:00:00

abstract：:A phosphorylcholine (PC)-binding IgG (Mab2) antibody produced by a hybridoma derived from a BALB/c mouse which had been immunized against Trichinella spiralis was found to bind to the immunizing antigen (TSC) but not to other PC-associated antigens such as pneumococcal antigen (PNC) and PC-conjugated ovalbumin (PC-OVA...

journal_title：Molecular immunology

authors： Lim PL,Leung DT,Chui YL,Ma CH

更新日期：1994-10-01 00:00:00

abstract：:The scid mutation interferes with normal rearrangement of antigen receptor genes, leading to an absence of T and B lymphocytes in most SCID mice. However, the SCID phenotype is "leaky", with an age- and strain-dependent increase in the incidence of mice with small number of T and B cells and readily detectable serum i...

journal_title：Molecular immunology

authors： Riggs JE,Feeney AJ,Kirven M,Mosier DE

更新日期：1994-08-01 00:00:00

abstract：:We have previously used immunoelectron microscopy (IEM) to generate a three-dimensional map of idiotypic (Id) and isotypic epitopes on the Fab arms of HGAC 39 (Roux et al., 1987, Proc. natn. Acad. Sci. U.S.A. 84, 4984-4988), a mouse IgG3 monoclonal antibody (Mab). In this report, we analyse the geometry of the various...

journal_title：Molecular immunology

authors： Roux KH,Greenspan NS

更新日期：1994-06-01 00:00:00

abstract：:In order to improve our knowledge of the human immunoglobulin variable lambda locus (IGLV), we mapped one cosmid clone (designated as C40.2) isolated by screening a Colo320HSR genomic library. The 34 kb insert of the C40.2 clone was shown to contain six genes. One gene, IGLV2S1, belongs to the V lambda II subgroup. Fo...

journal_title：Molecular immunology

authors： Frippiat JP,Lefranc MP

更新日期：1994-06-01 00:00:00

abstract：:In this paper we report a chemometric approach to Quantitative Structure-Activity Relationship (QSAR) analysis applied to a study of the binding of peptides to Major Histocompatibility Complex (MHC) class I proteins. Peptides which possess the known primary anchor residue motif for HLA-B27 binding do not necessarily b...

journal_title：Molecular immunology

authors： Rovero P,Riganelli D,Fruci D,Viganò S,Pegoŕaro S,Revoltella R,Greco G,Butler R,Clementi S,Tanigaki N

更新日期：1994-05-01 00:00:00

abstract：:Specific IgE antibodies have been affinity-purified from recombinant grass pollen allergens, and used to identify isoforms of the two major allergens of rye-grass pollen, Lol p I and Lol p V and cross-reactive allergens in other grasses. Lol p I-specific IgE (affinity-purified from the recombinant protein expressed by...

journal_title：Molecular immunology

authors： Smith PM,Ong EK,Knox RB,Singh MB

更新日期：1994-04-01 00:00:00

abstract：:Mouse monoclonal antibody AUK12-20 binds to human IL-6 receptor and inhibits IL-6 functions. It has been humanized by CDR-grafting for therapeutic use. In the design of reshaped human AUK12-20 VL region, the human framework regions (FRs) from the human Bence-Jones protein REI were used. The reshaped human AUK12-20 lig...

journal_title：Molecular immunology

authors： Sato K,Tsuchiya M,Saldanha J,Koishihara Y,Ohsugi Y,Kishimoto T,Bendig MM

更新日期：1994-04-01 00:00:00

abstract：:The binding of a T cell-presented peptide to MHC class II alpha,beta chains occurs as a concurrent process with the release of the associated invariant chain (Ii) by cathepsin B. Ii was digested by cathepsin B from solubilized, MHC class II alpha,beta,Ii complexes in the presence of N-hydroxysuccinimidyl-4-azidobenzoa...

journal_title：Molecular immunology

authors： Daibata M,Xu M,Humphreys RE,Reyes VE

更新日期：1994-03-01 00:00:00

abstract：:Mammalian B-cell specific somatic hypermutation contributes to affinity maturation of the antibody response. This mutator activity is highly focused on rearranged immunoglobulin variable regions, but the underlying mechanism remains to be elucidated. In an effort to gain insights into the mechanism of somatic hypermut...

journal_title：Molecular immunology

authors： Rogerson BJ

更新日期：1994-02-01 00:00:00

abstract：:Idiotypic-like interactions between mAbs directed against cytidine (Cyd) or guanosine (Guo) nucleosides were characterized. These mAbs, Cyd-1 (IgG2b, kappa), Guo-1 (IgG1, kappa) and Guo-2 (IgG1, kappa) were derived from splenocytes of A/J mice immunized with Cyd-KLH or Guo-KLH and recognized the nucleoside base moieti...

journal_title：Molecular immunology

authors： Cottet MH,Denoyelle C,Bordenave G

更新日期：1994-01-01 00:00:00

abstract：:Membrane immunoglobulins are integral proteins on B cell surfaces that bind foreign antigens and are critically involved in the regulation of the immune response. Based upon the model of serum IgG, it has been assumed that membrane immunoglobulins are essentially four chain disulfide-linked structures of the form H2L2...

journal_title：Molecular immunology

authors： Alhasan S,Santini R,Li Q,Rosenspire A

更新日期：1993-12-01 00:00:00